Development and testing of the Active Temperature, Ozone and Moisture Microwave Spectrometer (ATOMMS) cm and mm wavelength occultation instrument

We present initial results from testing a new remote sensing system called the Active Temperature, Ozone and Moisture Microwave Spectrometer (ATOMMS). ATOMMS is designed as a satellite-to-satellite occultation system for monitoring climate. We are developing the prototype instrument for an aircraft to aircraft occultation demonstration. Here we focus on field testing of the ATOMMS instrument, in particular the remote sensing of water by measuring the attenuation caused by the 22 GHz and 183 GHz water absorption lines. Our measurements of the 183 GHz line spectrum along an 820 m path revealed that the AM 6.2 spectroscopic model provdes a much better match to the observed spectrum than the MPM93 model. These comparisons also indicate that errors in the ATOMMS amplitude measurements are about 0.3%. Pressure sensitivity bodes well for ATOMMS as a climate instrument. Comparisons with a hygrometer revealed consistency at the 0.05 mb level, which is about 1% of the absolute humidity. Initial measurements of absorption by the 22 GHz line made along a 5.4 km path between two mountaintops captured a large increase in water vapor similar to that measured by several nearby hygrometers. A storm passage between the two instruments yielded our first measurements of extinction by rain and cloud droplets. Comparisons of ATOMMS 1.5 mm opacity measurements with measured visible opacity and backscatter from a weather radar revealed features simultaneously evident in all three datasets confirming the ATOMMS measurements. The combined ATOMMS, radar and visible information revealed the evolution of rain and cloud amounts along the signal path during the passage of the storm. The derived average cloud water content reached typical continental cloud amounts. These results demonstrated a significant portion of the information content of ATOMMS and its ability to penetrate through clouds and rain which is critical to its all-weather, climate monitoring capability

Ab interno trabecular bypass surgery with Schlemm´s canal microstent (Hydrus) for open angle glaucoma

Background: Glaucoma is a leading cause of irreversible blindness. A number of minimally‐invasive surgical techniques have been introduced as a treatment to prevent glaucoma from progressing; ab interno trabecular bypass surgery with the Schlemm's canal Hydrus microstent is one of them. / Objectives: To evaluate the efficacy and safety of ab interno trabecular bypass surgery with the Hydrus microstent in treating people with open angle glaucoma (OAG). / Search methods: On 7 May 2019, we searched CENTRAL (2019, Issue 5), which contains the Cochrane Eyes and Vision Trials Register; Ovid MEDLINE; Ovid Embase; the ISRCTN registry; ClinicalTrials.gov; and the WHO ICTRP. / Selection criteria: We searched for randomised controlled trials (RCTs) of the Hydrus microstent, alone or with cataract surgery, compared to other surgical treatments (cataract surgery alone, other minimally‐invasive glaucoma device techniques, trabeculectomy), laser treatment, or medical treatment. / Data collection and analysis: A minimum of three authors independently extracted data from reports of included studies, using a data collection form and analysed data, based on standard Cochrane methods. / Main results: We included three published studies, with 808 people randomised. Two studies had multiple international recruitment centres in the USA and other countries. The third study had several sites based in Europe. All three studies were sponsored by the Hydrus manufacturer Ivantis Inc. All studies included participants with mainly mild or moderate OAG (mean deviation between ‐3.6 dB (decibel) and ‐8.4 dB in all study arms), which was controlled with medication in many participants (mean medicated intraocular pressure (IOP) 17.9 mmHg to 19.1 mmHg). There were no concerns regarding allocation concealment bias, but masking of outcome assessors was high or unclear risk in all studies; masking of participants was achieved, and losses to follow‐up were not a concern. Two studies compared the Hydrus microstent combined with cataract surgery to cataract surgery alone, in participants with visually significant cataracts and OAG. We found moderate‐certainty evidence that adding the Hydrus microstent to cataract surgery increased the proportion of participants who were medication‐free from about half to more than three quarters at 12‐month, short‐term follow‐up (risk ratio (RR) 1.59, 95% confidence interval (CI) 1.39 to 1.83; 2 studies, 639 participants; I² = 0%; and 24‐month, medium‐term follow‐up (RR 1.63, 95% CI 1.40 to 1.88; 2 studies, 619 participants; I² = 0%). The Hydrus microstent combined with cataract surgery reduced the medium‐term mean change in unmedicated IOP (after washout) by 2 mmHg more compared to cataract surgery alone (mean difference (MD) ‐2.00, 95% CI ‐2.69 to ‐1.31; 2 studies, 619 participants; I² = 0%; moderate‐certainty evidence), and the mean change in IOP‐lowering drops (MD ‐0.41, 95% CI ‐0.56 to ‐0.27; 2 studies, 619 participants; I² = 0%; low‐certainty evidence). We also found low‐certainty evidence that adding a Hydrus microstent to cataract surgery reduced the need for secondary glaucoma surgery from about 2.5% to less than 1% (RR 0.17, 95% CI 0.03 to 0.86; 2 studies, 653 participants; I² = 27%; low‐certainty evidence). Intraocular bleeding, loss of 2 or more visual acuity (VA) lines, and IOP spikes of 10 mmHg or more were rare in both groups; estimates were imprecise, and included both beneficial and harmful effects. There were no cases of endophthalmitis in either group. No data were available on the proportion of participants achieving IOP less than 21 mmHg, 17 mmHg, or 14 mmHg; health‐related quality of life (HRQOL), or visual field progression. One study provided short‐term data for the Hydrus microstent compared with the iStent trabecular micro‐bypass stent (iStent: implantation of two devices in a single procedure) in 152 participants with OAG (148 in analyses). Use of the Hydrus increased the proportion of medication‐free participants from about a quarter to about half compared to those who received iStent, but this estimate was imprecise (RR 1.94, 95% CI 1.21 to 3.11; low‐certainty evidence). Use of the Hydrus microstent reduced unmedicated IOP (after washout) by about 3 mmHg more than the iStent (MD ‐3.10, 95% CI ‐4.17 to ‐2.03; moderate‐certainty evidence), and the use of IOP‐lowering medication (MD ‐0.60, 95% CI ‐0.99 to ‐0.21; low‐certainty evidence). Both devices achieved a final IOP < 21 mmHg in most participants (Hydrus microstent: 91.8%; iStent: 84%; RR 1.09, 95% CI 0.97 to 1.23; low‐certainty evidence). None of the participants who received the Hydrus microstent (N = 74) required additional glaucoma surgery; two participants who received the iStent (N = 76) did. Few adverse events were found in either group. No data were available on the proportion of participants achieving IOP less than 17 mmHg or 14 mmHg, or on HRQOL. / Authors' conclusions: In people with cataracts and generally mild to moderate OAG, there is moderate‐certainty evidence that the Hydrus microstent with cataract surgery compared to cataract surgery alone, likely increases the proportion of participants who do not require IOP lowering medication, and may further reduce IOP at short‐ and medium‐term follow‐up. There is moderate‐certainty evidence that the Hydrus microstent is probably more effective than the iStent in lowering IOP of people with OAG in the short‐term. Few studies were available on the effects of the Hydrus microstent, therefore the results of this review may not be applicable to all people with OAG, particularly in selected people with medically uncontrolled glaucoma, since IOP was controlled with medication in many participants in the included studies. Complications may be rare using the Hydrus microstent, as well as the comparator iStent, but larger studies are needed to investigate its safety

Retrieval of water vapor using ground-based observations from a prototype ATOMMS active centimeter- and millimeter-wavelength occultation instrument

A fundamental goal of satellite weather and climate observations is profiling the atmosphere with in situ-like precision and resolution with absolute accuracy and unbiased, all-weather, global coverage. While GPS radio occultation (RO) has perhaps come closest in terms of profiling the gas state from orbit, it does not provide sufficient information to simultaneously profile water vapor and temperature. We have been developing the Active Temperature, Ozone and Moisture Microwave Spectrometer (ATOMMS) RO system that probes the 22 and 183&thinsp;GHz water vapor absorption lines to simultaneously profile temperature and water vapor from the lower troposphere to the mesopause. Using an ATOMMS instrument prototype between two mountaintops, we have demonstrated its ability to penetrate through water vapor, clouds and rain up to optical depths of 17 (7 orders of magnitude reduction in signal power) and still isolate the vapor absorption line spectrum to retrieve water vapor with a random uncertainty of less than 1&thinsp;%. This demonstration represents a key step toward an orbiting ATOMMS system for weather, climate and constraining processes. ATOMMS water vapor retrievals from orbit will not be biased by climatological or first-guess constraints and will be capable of capturing nearly the full range of variability through the atmosphere and around the globe, in both clear and cloudy conditions, and will therefore greatly improve our understanding and analysis of water vapor. This information can be used to improve weather and climate models through constraints on and refinement of processes affecting and affected by water vapor.</p

The Cosmic Background Imager

Design and performance details are given for the Cosmic Background Imager (CBI), an interferometer array that is measuring the power spectrum of fluctuations in the cosmic microwave background radiation (CMBR) for multipoles in the range 400 < l < 3500. The CBI is located at an altitude of 5000 m in the Atacama Desert in northern Chile. It is a planar synthesis array with 13 0.9-m diameter antennas on a 6-m diameter tracking platform. Each antenna has a cooled, low-noise receiver operating in the 26-36 GHz band. Signals are cross-correlated in an analog filterbank correlator with ten 1 GHz bands. This allows spectral index measurements which can be used to distinguish CMBR signals from diffuse galactic foregrounds. A 1.2 kHz 180-deg phase switching scheme is used to reject cross-talk and low-frequency pick-up in the signal processing system. The CBI has a 3-axis mount which allows the tracking platform to be rotated about the optical axis, providing improved (u,v) coverage and a powerful discriminant against false signals generated in the receiving electronics. Rotating the tracking platform also permits polarization measurements when some of the antennas are configured for the orthogonal polarization.Comment: 14 pages. Accepted for publication in PASP. See also http://www.astro.caltech.edu/~tjp/CBI

Revealing Polylepis microphylla as a suitable tree species for dendrochronology and quantitative wood anatomy in the Andean montane forests

In the tropical Andes climate change is expected to increase temperatures and change precipitation patterns. To overcome the lack of systematic weather records that limits the performance of climate models in this region, the use of the environmental information contained in tree rings from tropical Andean species have been found useful to reconstruct spatio-temporal climate variability. Because classical dendrochronology based on ring-width patterns is often challenging in the tropics, alternative approaches such as Quantitative Wood Anatomy (QWA) based on the measurement and quantification of anatomical traits within tree rings can be a significant advance in the field. Here we assess the dendrochronological potential of Polylepis microphylla and its climate sensitivity by using i) classic dendrochronological methods to generate the first Tree-ring Width (TRW) chronology for this tree species spanning from 1965 to 2018; ii) radiocarbon (¹⁴C) analyses as an independent validation method to assess the annual periodicity of the tree growth layers; and iii) QWA to generate tree-ring annual records of the number (VN) and size (VS) of vessels to investigate the climate sensitivity of these anatomical traits. The annual periodicity in P. microphylla radial growth was confirmed by both dendrochronological and ¹⁴C analyses. We found that VN and VS are promising new proxies to reconstruct climate variability in this region and that they provide different information than TRW. While TRW provides information at inter-annual resolution (i.e., year-to-year variability), VN and VS generated with sectorial QWA provide intra-annual resolution for each stage of the growing process. The TRW and the anatomical traits (i.e., VN and VS) showed strong positive correlation with maximum temperature for different periods of the growing season: while VS is higher with warmer conditions prior to the growing season onset, tree-rings are wider and present higher number of vessels when warmer conditions occur during the current growing season. Our findings pointed out the suitability of P. microphylla for dendrochronological studies and may suggest a good performance of this species under the significant warming expected according to future projections for the tropical Andes.Fil: Rodríguez Morata, C.. Columbia University; Estados UnidosFil: Pacheco Solana, A.. Columbia University; Estados UnidosFil: Ticse Otarola, Ginette Vilma Alicia. Universidad Continental; Perú. Asociación ANDINUS; PerúFil: Boza Espinoza, T. E.. Pontificia Universidad Católica de Perú; PerúFil: Crispín-DelaCruz, D.B.. Universidad Federal Rural Pernambuco; Brasil. Universidad Continental; PerúFil: Santos, G. M.. University of California; Estados UnidosFil: Morales, Mariano Santos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto Argentino de Nivología, Glaciología y Ciencias Ambientales. Provincia de Mendoza. Instituto Argentino de Nivología, Glaciología y Ciencias Ambientales. Universidad Nacional de Cuyo. Instituto Argentino de Nivología, Glaciología y Ciencias Ambientales; Argentina. Universidad Continental; PerúFil: Requena Rojas, Edilson Jimmy. Universidad Continental; PerúFil: Andreu Hayles, Laia. Institució Catalana de Recerca I Estudis Avançats; España. Consejo Superior de Investigaciones Científicas. Centre de Recerca Ecológica I Aplicacions Forestals; España. Columbia University; Estados Unido

Optical-to-NIR magnitude measurements of the Starlink LEO Darksat satellite and effectiveness of the darkening treatment

Four observations of Starlink's LEO communication satellites, Darksat and STARLINK-1113, were conducted on two nights with two telescopes. The Chakana 0.6\,m telescope at the Ckoirama observatory (Chile) observed both satellites on 5\,Mar\,2020 (UTC) and 7\,Mar\,2020 (UTC) using a Sloan r' and Sloan i' filter, respectively. The ESO VISTA 4.1\,m telescope with the VIRCAM instrument observed both satellites on 5\,Mar\,2020 (UTC) and 7\,Mar\,2020 (UTC) in the NIR J-band and Ks-band, respectively. The calibration, image processing, and analysis of the Darksat images give r\,$\approx$\,5.6\,mag, i\,$\approx$\,5.0\,mag, J\,$\approx$\,4.2\,mag, and Ks\,$\approx$\,4.0\,mag when scaled to a range of 550\,km (airmass $=1$) and corrected for the solar incidence and observer phase angles. In comparison, the STARLINK-1113 images give r\,$\approx$\,4.9\,mag, i\,$\approx$\,4.4\,mag, J\,$\approx$\,3.8\,mag, and Ks\,$\approx$\,3.6\,mag when corrected for range, solar incidence, and observer phase angles. The data and results presented in this work show that the special darkening coating used by Starlink for Darksat has darkened the Sloan r' magnitude by 50\,\%, Sloan i' magnitude by 42\,\%, NIR J magnitude by 32\,\%, and NIR Ks magnitude by 28\,\%. The results show that both satellites increase in reflective brightness with increasing wavelength and that the effectiveness of the darkening treatment is reduced at longer wavelengths. This shows that the mitigation strategies being developed by Starlink and other LEO satellite operators need to take into account other wavelengths, not just the optical. This work highlights the continued importance of obtaining multi-wavelength observations of many different LEO satellites in order to characterise their reflective properties and to aid the community in developing impact simulations and developing mitigation tools.Comment: Accepted for publication in A&A, 10 pages, 10 figures, 3 table

Human cell types important for Hepatitis C Virus replication in vivo and in vitro. Old assertions and current evidence

Hepatitis C Virus (HCV) is a single stranded RNA virus which produces negative strand RNA as a replicative intermediate. We analyzed 75 RT-PCR studies that tested for negative strand HCV RNA in liver and other human tissues. 85% of the studies that investigated extrahepatic replication of HCV found one or more samples positive for replicative RNA. Studies using in situ hybridization, immunofluorescence, immunohistochemistry, and quasispecies analysis also demonstrated the presence of replicating HCV in various extrahepatic human tissues, and provide evidence that HCV replicates in macrophages, B cells, T cells, and other extrahepatic tissues. We also analyzed both short term and long term in vitro systems used to culture HCV. These systems vary in their purposes and methods, but long term culturing of HCV in B cells, T cells, and other cell types has been used to analyze replication. It is therefore now possible to study HIV-HCV co-infections and HCV replication in vitro

Intracellular Calcium and Sodium Modulation of Self-Assembled Neocartilage Using Costal Chondrocytes

Ion signaling through Ca2+ and Na+ plays a key role in mechanotransduction and encourages a chondrogenic phenotype and tissue maturation. In this study, we propose that the pleiotropic effects of Ca2+ and Na+ modulation can be used to induce maturation and improvement of neocartilage derived from redifferentiated expanded chondrocytes from minipig rib cartilage. Three ion modulators were employed: (1) 4α-phorbol-12,13-didecanoate (4-αPDD), an agonist of the Ca2+-permeable transient receptor potential vanilloid 4 (TRPV4), (2) ouabain, an inhibitor of the Na+/K+ pump, and (3) ionomycin, a Ca2+ ionophore. These ion modulators were used individually or in combination. While no beneficial effects were observed when using combinations of the ion modulators, single treatment of constructs with the three ion modulators resulted in multiple effects in structure-function relationships. The most significant findings were related to ionomycin. Treatment of neocartilage with ionomycin produced 61% and 115% increases in glycosaminoglycan and pyridinoline crosslink content, respectively, compared with the control. Moreover, treatment with this Ca2+ ionophore resulted in a 45% increase of the aggregate modulus, and a 63% increase in the tensile Young's modulus, resulting in aggregate and Young's moduli of 567 kPa and 8.43 MPa, respectively. These results support the use of ion modulation to develop biomimetic neocartilage using expanded redifferentiated costal chondrocytes. Impact Statement New cost-effective, replicable, and highly controllable strategies are required to develop neocartilage with biomimetic properties akin to native tissue. Ion signaling plays a key role in mechanotransduction, promoting chondrogenic phenotype. Using rib cartilage, we proposed that Ca2+ and Na+ modulation could be used to induce maturation of neotissue derived from redifferentiated, expanded costal chondrocytes, improving its mechanical properties. Our results indicate that Ca2+ modulation with ionomycin, which stimulated extracellular matrix deposition and collagen crosslinking, improved morphological and mechanical features of neocartilage constructs, and holds potential as a powerful tool to engineer hyaline-like tissues